Brazzein

Type of Protein	Sweet
Protein name	Brazzein
Companies producing this protein	Perfect Day has recieved GRAS notice Sweegen 21st.Bio Levprot Bioscience Oobli Bestzyme
Function (dairy alternative, colorant, texture, taste...)	Sweet taste (Hellekant, 2005)
Target application (cheese, infant formula, meat...)	Sweetener in beverages & food (Hellekant, 2005)
Purity requirement/purification values	Has been isolated at 98% purity at pilot scale (Carlson et al., 2014) >90% pure in the application of Perfect Day
Techno-functional properties	Thermostable & water soluble (Saraiva et al., 2023)
Nutritional properties	Low-calorie (Levprot Bioscience, n.d.)
Advantages	Can be used in combination with othger sweeteners to decrease their side-taste. It has the mosty sugar-like taste of all sweet proteins (Saraiva et al., 2023). Tested on animal models and concluded to be safe, showed no allergenic, cytoogenic or mutagenic properties (Novik et al., 2023)
Challenges/risk factors	Contains four disulphide bonds that have to be folded correctly for the protein to be funcional. Certain host strains might struggle with this (Farag et al., 2022).

Komagatella phaffi (Pichia pastoris)

Kluyveromyces lactis